Confocal Microscopy (SAI-MICROCON)

Personal

Sonia Moraleja Budia (technician).

Contacto:

Servicios

1. Acquisition of simple images with one or two fluorochromes.
2. Characterisation of three-dimensional structures by fluorescence.
3. Advance fluorescence microscopy techniques: FRET, FRAP.
4. LIGHTNING detection technology to obtain high-resolution confocal images, fast and in real time (120nm).

Equipamientos

• Leica Stellaris 8 Confocal Microscope:
  • 2 PMT fluorescence detectors
  • 5 laser lines:
    • Argón 65 mW Laser with 3 lines:
      • 457 nm
      • 488 nm
      • 514 nm
    • DPSS 20 mW Laser: 561 nm
  • HeNe 10 mW Laser: 633 nm
• LIGHTNING – Super resolution 120nm.
• Lenses:
  • 10X Plan Apo CS2
  • 40X Plan Apo oil CS2
  • 63X Plan Apo oil CS2

Working station with monitor and assistant software modules for FRET and FRAP and 3D viewer.

Tarifas

• PDF